Thursday's trial at Caulfield Heath had been in preparation for a trip to Brisbane where Without A Fight held an entry in the Group 1 Doomben Cup (2000m) on Saturday week.
"He has been an absolute gentleman to have in the stable and will be remembered as one of the finest stayers of his generation," the Freedman's said in a statement.
"His Cups double not only delivered the pinnacle of our training careers but also created unforgettable memories for all involved – from our dedicated track riders, stable hands, vets, farriers, to those at the spelling farms.
"He has left a lasting impression on everyone fortunate enough to be a part of his journey.
"We extend our heartfelt thanks to his owner, Sheikh Mohammed Obaid Al Maktoum, for entrusting us with the privilege of training such a remarkable horse in Australia. We are truly indebted to him for the opportunity.
"As Without A Fight begins the next chapter of his life, we wish him the very best in his retirement.
"He has given us all so much – and for that, we are forever grateful."
Without A Fight retires having registered 11 wins from his 24 starts with career prize money of just over $9.7 million.
